Identification and Structural Elucidation by Amino Acid Sequencing of Peptide Impurities by 2DlC-MS/MS

Peptide impurity profiles require increasingly complex separation methods in order to detect and adequately quantitate peptide impurities. Mobile phase conditions optimal for these separations in most cases consist of non-volatile salt based buffer systems that are not inherently LC/MS compatible. 2D-LC has been demonstrated to overcome this issue by extracting the impurity peaks of interest and transferring them to a mass spectrometry compatible solvent system for further analysis.
This webinar demonstrates that a 2D-LC system when paired with a Q-TOF mass spectrometer can automate impurity extraction by 2D-LC to facilitate identification by 2D-LC/MS and also further structural elucidation by 2D-LC MS/MS.